The Multiple Myeloma Minimal Residual Disease Panel (MM-MRD) is comprised of a pre-mixed 6-color 8-antibody combination and two single antibody vials : CD138-BV421 and CD27-BV510. The panel is designed for the qualitative and semi-quantitative identification and discrimination of malignant and normal plasma cells by flow cytometry using BD FACSCanto™ II and/or BD FACSLyric™ Flow Cytometers. It is intended to be used for staining of bone marrow specimens of Multiple Myeloma treated patients.
The Multiple Myeloma Minimal Residual Disease Panel recignizes the following antigens: CD138, CD27, CD38, CD45, CD56, CD19, CD81, CD117, cytoplasmic IgKappa and IgLambda.
The kit is composed of
- Lyophilized Antibody Combination Tube 1:
- Four (4) lyophilized vials of 5 tests for surface staining that contain:
- Anti-human CD38-FITC antibody, multi-epitope.
- Anti-human CD56-PE antibody, clone: C5.9, isotype: IgG2b.
- Anti-human CD45-PerCP-Cyanine 5.5 antibody, clone: EO1, isotype: IgG2a.
- Anti-human CD19-PE-Cyanine7 antibody, clone: SA287, isotype: IgG2a.
- Anti-human CD117-APC antibody, clone: 104D2, isotype: IgG1.
- Anti-human CD81-APC-C750™ antibody, clone: M38, isotype: IgG1.
- Four (4) lyophilized vials of 5 tests for surface staining that contain:
- Lyophilized Antibody Combination Tube 2:
- Four (4) lyophilized vials of 5 tests each for surface staining that contain:
- Anti-human CD38-FITC antibody, multi-epitope.
- Anti-human CD56-PE antibody, clone: C5.9, isotype: IgG2b.
- Anti-human CD45-PerCP-Cyanine 5.5 antibody, clone: EO1, isotype: IgG2a.
- Anti-human CD19-PE-Cyanine7 antibody, clone: SA287, isotype: IgG2a.
- Four (4) lyophilized vials of 5 tests each for cytoplasmic staining that contain:
- Anti-human CyIgκ-APC goat antibody, clone: polyclonal.
- Anti-human CyIgλ-APC-C750™ goat antibody, clone: polyclonal.
- Four (4) lyophilized vials of 5 tests each for surface staining that contain:
- Antibody vials in liquid format:
- Two (2) liquid antibody vials of:
- Anti-human CD138-BV421 antibody, clone: MI15, isotype: IgG1
- Anti-human CD27-BV510 antibody, clone: O323, isotype: IgG1
- Two (2) liquid antibody vials of:
This product should be used in combination with the EuroFlow™ Standard Operation Procedures for instrument setup:
- The protocol for BD FACSCanto™ II “EuroFlow SOP for Instrument set-up and compensation Version 1.2.1 – 29 October 2019” can be downloaded here.
- The protocol for BD FACSLyric™ “EuroFlow SOP for instrument set-up and compensation for BD Lyric instrument for the 8-color panels Version 1.8 – 1 July 2019” can be downloaded here.
